Separation of mAbs molecular variants by analytical hydrophobic interaction chromatography HPLC: overview and applications

Separation of mAbs molecular variants by analytical hydrophobic interaction chromatography HPLC: overview and applications

Hydrophobic interaction chromatography-high performance liquid chromatography (HIC-HPLC) is a powerful analytical method used for the separation of molecular variants of therapeutic proteins. The method has been employed for monitoring various post-translational modifications, including proteolytic fragments and domain misfolding in etanercept (Enbrel®); tryptophan oxidation, aspartic acid isom...

Hydrophobic Interaction Chromatography

Hydrophobic Interaction Chromatography: Fundamentals and Applications in Biomedical Engineering

Hydrophobic interaction chromatography (HIC) a powerful technique used for separation and purification of biomolecules. It was described for the first time by Shepard & Tiselius (1949), using the term “salting-out chromatography”. Later, Shaltiel & Er-el (1973) introduced the term “hydrophobic chromatography”. Finally, Hjerten (1973) described this technique as “hydrophobic interaction chromato...

Hydrophobic Interaction Chromatography

modeling for the design of a Biomimetic chimeric ligand. Application to the puriRcation of bovine heart L-lactate dehydrogenase. Biotechnology and Bioengineering 63: 321}331. Lowe CR (1984) Applications of reactive dyes in biotechnology. In: Wiseman A (ed.) Topics in Enzyme and Fermentation Biotechnology, vol. 9. Chichester: Ellis Horwood. Lowe CR, Burton S, Pearson J, Clonis YD and Stead CV (1...

Hydrophobic Interaction Chromatography.

Most proteins and large polypeptides have hydrophobic regions at their surface. These hydrophobic "patches" are due to the presence of the side chains of hydrophobic or nonpolar amino acids such as phenylalanine, tryptophan, alanine, and methionine. These surface hydrophobic regions are interspersed between more hydrophilic or polar regions and the number, size, and distribution of them is a sp...